ABSTRACT
Object To study the chemical structures and bioactivity of the water-soluble constituents from Polygonum cuspidatum Sieb. et Zucc. Methods To isolate the constituents by reverse phase methods, and characterize their structures by the analysis of chemical property and spectral data. Results Six compounds were isolated from the 60% aqueous acetone extract from the rhizome of P. cuspidatum. Their structures were elucidated as reveratrol (Ⅰ); piceid (Ⅱ); 2, 3-dihydro-2-(4′-O-?-D-glucopyranosyl-3′-methoxy-phenyl)-3-hydroxymethyl-5-(3-hydroxypropyl)-7-methoxybenzofuran (Ⅲ); 2, 6-dimethoxy-p-hydroquinone-1-O-?-D-glucopyranoside (Ⅳ); 5, 7-dihydroxy-isobenzofuran (Ⅴ) and 5, 7-dihydroxy-isobenzofuran-7-O-?-D-glucopyranoside(Ⅵ), respectively. Conclusion Compounds Ⅲ-Ⅵ are isolated from the plant for the first time. Compounds Ⅰ-Ⅵ show no DNA cleavage activity. Compound Ⅱ exhibits weak cytotoxicity against two human cancer cell lines (KB and MCF-7) in vitro.
ABSTRACT
Object To study the chemical structures and DNA cleavage activity of the water-soluble constituents from Polygonum bistorta L.Methods To isolate the constituents by reverse phase chromatography, and characterize their structures by the analysis of chemical property and spectral data.Results Ten compounds were isolated from the 60% acetone extract of the rhizoma from P.bistorta.Their structures were elucidated as gallic acid (Ⅰ), tryptophan (Ⅱ), 2, 6-dihydroy-bezoic acid (Ⅲ), (+)-catechin (Ⅳ), chlorogenic acid (Ⅴ), (-)-epicatechin-5-O-?-D-glucopyranoside (Ⅵ), (+)-catechin-7-O-?-D-glucopyranoside (Ⅶ), 1-(3-O-?-D-glucopyranosyl-4, 5-dihydroxy-phenyl)-ethanone (Ⅷ), (+)-catechin-5-O-?-D-glucopyranoside (Ⅸ) and (-)-epicatechin (Ⅹ), respectively.Conclusion Compounds Ⅱ, Ⅲ, Ⅴ-Ⅹ were isolated from the plant for the first time.Compounds Ⅰ, Ⅳ, Ⅵ, Ⅶ, Ⅸ, Ⅹ showed significant DNA cleavage activity.